Establishment of Vibrio parahaemolyticus growth predictive model by Real-time quantification PCR on ready-to-eat Shrimp

The object was to establish growth predictive model of Vibrio parahaemolyticus on ready-to-eat shrimp by Real-time quantification PCR method. Firstly we constructed the standard plasmid and established the standard curve by 10-fold dilution. Secondly we detected the number of Vibrio parahaemolyticus on shrimp by real-time qPCR method. At last we established the growth predictive model of Vibrio parahaemolyticus on shrimp stored at 37℃ . We compared this model with a traditional predictive model which used the data obtained by construction of microbiological methods. The results showed that it could establish Gmopertz model, Logistic model and Richards model based on the real -time qPCR method and construction of microbiological method (R2 >0.9) . Establishment of a microbial predictive model by real-time qPCR method will be a trend of predictive microbiology field in the future, because of the advantage of real-time qPCR methods such as time-saving, labar-saving and accurate.