Study on the determination of ferulic acid esterase enzyme activity with spectrophotometer method and its impact factors

The UV-Vis spectrophotometer methods to determination the ferulic acid esterase enzyme activity was studied using destarchedwheat bran (DSWB) and 4-nitrophenyl ferulate as the substrates respectively.The enzyme activity was calculated by measuring the quantities of ferulic acid released and the quantities of p-nitrophenol released.The factors influencing the enzyme activity determination were also investigated.The method for the determination the ferulic acid esterase enzyme activity using DWB substrate was validated with the average recovery of 99.86% and RSD was 0.65% . It was compared with the method of 4-nitrophenyl ferulate spectrophotometer and the method of HPLC.The results showed that the DSWB spectrophotometer method had advantages of a good stability, smaller relative deviation, simple and feasible.