Study on preparation and antioxidant activity of the Seleno-polysaccharides from Tussilago farfara

To optimize the selenizing modification conditions of Tussilago farfara polysaccharides and its antioxidant activity were studied. Polysaccharides from Tussilago farfara was selenized as the sodium selenite method. According to the content of selenite, response surface methodology was operated to optimizing the selenizing modificiation.The effects of selenizing Tussilago farfara polysaccharides (STFPs) on scavenging DPPH was determined.The result showed that 1.06 ∶ 1 of fraction of selenite reagents, 70℃, 11h, 0.5% of volume fraction of HNO3 were the optimum conditions.The average content of selenium in STFPs was 3.84mg /g.Compared with TFPs, the effect of STFPs had been improved on scavenging DPPH, O-2, OH·and the most effect of DPPH·scavenging.