Influence of bovine serum albumin-assisted strategy on preparation of cross-linked enzyme aggregates of Phenylalanine ammonia lyase

Cross-linked enzyme aggregates of phenylalanine ammonia lyase (BSA-PAL-CLEAs) were prepared by using BSA as an additive.Conditions for the preparation of BSA-PAL-CLEAs were optimized, and the stability of free PAL, PAL-CLEAs and BSA-PAL-CLEAs was also evaluated, respectively.The optimized conditions of BSA-PAL-CLEAs preparation were as follows: 80%-saturation ammonia sulfate, 5mg/mL BSA, 10μL glutaraldehyde (10% v/v) and 2h cross-linking time.Under optimized conditions, the activity recovery of prepared BSA-PAL-CLEAs was 1.92-fold than that of PAL-CLEAs.Moreover, the thermal stability and storage stability of BSA-PAL-CLEAs was higher than that of PAL-CLEAs.However, stability against pH of free PAL、PAL-CLEAs and BSA-PAL-CLEAs was similar. In addition, compared with PAL-CLEAs, the stability of BSA-PAL-CLEAs against organic solvents was decreased.