Preliminary study on the viable but non-culturable state of Lactobacillus plantarum

The 16S rDNA sequence of an unidentified lactic acid bacteria was measured by 16S rDNA sequence analysis method,and compared the gene sequences homology with the gene pool,and the bacterium was identified as Lactobacillus plantarum. Conditions to induce Lactobacillus plantarum into viable but non-culturable(VBNC) and the resuscitation conditions were studied. Results showed that when the bacterium was induced in the condition of aerobic,-20℃,in MRS liquid medium,pH5.5～6.2 the bacterium entered VBNC state in 12d. Lactobacillus plantarum could resuscitate from VBNC state when it was incubated in MRS liquid medium added with 6% tween-80 and the MRS liquid medium added with nothing in 48h and 96h respectively.