Optimization of microwave-assisted alkaline extraction condition of protein from peanut meal

Peanut protein was obtained through microwave -assisted alkaline extraction and acid precipitation from peanut meal. Compared with alkali extraction, peanut protein extraction yield could be enhanced by 18.00% through microwave assisted method, and the effect was remarkable. Four extraction parameters including ratio of material and liquid, pH, microwave extraction power and microwave extraction time were optimized by orthogonal experiment based on single factor investigation. The influences of these factors on different degree was an order of microwave extraction power>pH>microwave extraction time>ratio of material and liquid. The optimum conditions of microwave-assisted alkaline extraction were as follows:ratio of material and liquid 1∶10 (g/mL) , pH10.0, microwave extraction power 480W and microwave extraction time 4 minutes. Under these conditions, the maximum protein extraction rate of 85.43% was obtained.