Science and Technology of Food Industry

ISSN 1002-0306
CN   11-1759/TS
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Optimization of extraction process of hyaluronidase from bovine liver[J]. Science and Technology of Food Industry, 2013, (16): 234-238. DOI: 10.13386/j.issn1002-0306.2013.16.081
Citation: Optimization of extraction process of hyaluronidase from bovine liver[J]. Science and Technology of Food Industry, 2013, (16): 234-238. DOI: 10.13386/j.issn1002-0306.2013.16.081
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Optimization of extraction process of hyaluronidase from bovine liver

    Abstract
  • As the raw material, hyaluronidase was isolated from bovine liver, the optimistic conditions was investigated. Plackett -Burman experiment, steepest ascent experiment and Central composite design were performed. The results showed that the optimistic isolation parameter were pH4.69 of acetate buffer, 0.22mol/L NaCl and extraction time 10.82h. Under these conditions, the specific activity of hyaluronidase from bovine liver reached 33.27U/g. By ammonia sulfate salting-out and Sephacryl S-100 preliminary purification, the specific activity reached 452.81U/g.
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