Subcritical acetone extraction of antioxidative components from marigold grounds

The subcritical acetone extraction method was used to extract antioxidative substances from marigold grounds. The effect of the concentration of subcritical acetone, extraction temperature, time and liquid to solid ratio on the extraction efficiency was investigated. The result showed that the content of total phenols and flavonoids of the marigold grounds extracts were generally increased with the elevating concentration of acetone, temperature, time and liquid to solid ratio at first, but the decreased instead. The best extraction condition of total phenols and flavonoids was 60% acetone, 140℃ and 30min. The yield of total flavonoids was15.03%±0.04%, the yield of total phenols was 13.12%±0.07%, the ABTS+· scavenging capacity was (3.18±0.07) mmol TE/g, the DPPH· scavenging capacity was (3.42 ±0.12) mmol TE/g. Compared with traditional solvent oscillation extraction and subcritical water, subcritical ethanol methods, subcritical acetone extraction had obvious advantages.