Science and Technology of Food Industry

ISSN 1002-0306
CN   11-1759/TS
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LI Zhi-jian, LI Gao-yang, ZHANG Qun, LIU Wei. Determination of L-citrulline in watermelon by high performance liquid chromatography with PITC precolumn derivation[J]. Science and Technology of Food Industry, 2013, (20): 53-55. DOI: 10.13386/j.issn1002-0306.2013.20.017
Citation: LI Zhi-jian, LI Gao-yang, ZHANG Qun, LIU Wei. Determination of L-citrulline in watermelon by high performance liquid chromatography with PITC precolumn derivation[J]. Science and Technology of Food Industry, 2013, (20): 53-55. DOI: 10.13386/j.issn1002-0306.2013.20.017
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Determination of L-citrulline in watermelon by high performance liquid chromatography with PITC precolumn derivation

    Abstract
  • A method of RP-HPLC with phenylisothiocyanate (PITC) precolumn derivatization was established for the determination of L-citrulline in watermelon. The calibration curves of L-citrulline were linear over the range of 4.26213.2μg/mL (R=0.9998) and the recovery rate was 98.1%. The content ranges of L-citrulline in watermelon were 1.132.71mg/g. The detection limit of the method was 1.1μg/mL. The method descried in this paper was fast, sensitive and reliable and it was most suitable for quantitative determination of L-citrulline in watermelon.
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