Extraction of flavonoids from Potentilla fulgens by microwave method using ethanol as extractant and evaluation of antioxidant activity by cell model

Flavonoids from Potentilla fulgens were extracted by microwave method. The effects of ethanol concentration in extractant, ratio of material to liquid, extraction temperature, microwave power and extraction duration on flavonoids yields were determined. The results showed that the optimum conditions were ethanol concentration in extractant 50%, ratio of material to liquid 1 ∶ 30, extraction temperature 60℃, microwave power 400W, and extraction duration 5min. The best yield of flavonoids was 5.68%. Furthermore, the flavonoids from P.fulgens showed obvious antioxidant activity in colon cancer cell ( HT- 29) and mice macrophage ( RAW264.7) , which were concentration-dependent.