Science and Technology of Food Industry

ISSN 1002-0306
CN   11-1759/TS
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YAO Lin-feng, HE Qiang. Interaction between tannic acid and pancreatic α-amylase[J]. Science and Technology of Food Industry, 2014, (03): 63-66. DOI: 10.13386/j.issn1002-0306.2014.03.040
Citation: YAO Lin-feng, HE Qiang. Interaction between tannic acid and pancreatic α-amylase[J]. Science and Technology of Food Industry, 2014, (03): 63-66. DOI: 10.13386/j.issn1002-0306.2014.03.040
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Interaction between tannic acid and pancreatic α-amylase

    Abstract
  • The inhibitory effect on porcine pancreaticα- amylase ( PPA) and its inhibitory mechanism of tannic acid ( TA) was investigated by UV-vis absorption and fluorescent spectrometry.As a non-competitive inhibitor, tannic acid showed significant inhibitory effect on PPA.The TA- PPA interaction was a spontaneous molecular process, and the TA- PPA binding site was closer to PPA trytophan residues. The UV absorbance at 270nm of PPA decreased and the absorption bands showed red-shift when it interacted with tannic acid.In addition, the intrinsic fluorescence of PPA could be significantly quenched by tannic acid.
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