Preparation of Antarctic krill peptides with anti-oxidant activity

In the study, the free radical scavenging ability against superoxide, DPPH and hydroxyl radical was investigated to screen optimum hydrolysis conditions for preparing Antarctic krill peptides with high anti- oxidant activity.Results indicated that peptides prepared by trypsin and neutral protease for 6h, showed the highest anti- oxidant activity, with free radical scavenging effect on superoxide, DPPH and hydroxyl radical by 11.5%, 72.7% and 84.8%, respectively.Through ultra- filtration, peptides ( 5 ~ 10ku) exhibited stronger scavenging activity against free radicals, which further revealed with two bands in the tricine-SDS-PAGE electrophoretogram.