Purification and determination of physicochemical property of Tuber indicum polysaccharide

The crude polysaccharide was extracted from the India truffle fruit body produced in Yunnan by the water extraction and alcohol sink method. After the separation of the Savage deproteinization and the DEAE cellulose column chromatography, three components: PTI-1, PTI-2, PTI-3 were obtained, among which PTI-2 was further purified by sephadex G-100 gel chromatography.And then a single polysaccharide called PTI-2A was got and the basal physicochemical properties of it was measured.The result showed that molecular weight of PTI-2A was 193388.2u and it was easily dissolving in water, dilute acid, dilute alkalis, but insoluble in organic solvent like methanol, acetone, chloroform, diethyl ether and dimethyl sulfoxide, etc., and it didn't contain protein, nucleic acid, phenols, amylum and free monosaccharide.