Optimization of the extraction technology of glycoprotein in nidamental gland from squid by response surface methodology

WANG Qian; LIU Shu-ji; LIN Cai-ping; WU Cheng-ye

doi:10.13386/j.issn1002-0306.2014.03.067

WANG Qian, LIU Shu-ji, LIN Cai-ping, WU Cheng-ye. Optimization of the extraction technology of glycoprotein in nidamental gland from squid by response surface methodology[J]. Science and Technology of Food Industry, 2014, (03): 261-265. DOI: 10.13386/j.issn1002-0306.2014.03.067

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Optimization of the extraction technology of glycoprotein in nidamental gland from squid by response surface methodology

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Abstract

Using the nidamental gland of squid as raw material, extraction of glycoprotein from nidamental gland of squid was optimized by response surface methodology based on single factor experiment.The results showed that the optimal extraction conditions were as follows: extraction time 3.5h, extraction temperature 25℃, NaOH concentration 0.37mol /L. Under these conditions, the yield of glycoprotein could reach 12.79%, which contained 72.5% of sugar, 22.7% of protein, and 3.0% of sulfate radical.The extracted glycoprotein from nidimental gland of squid is a kind of sulfating mucin.

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Optimization of the extraction technology of glycoprotein in nidamental gland from squid by response surface methodology

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