Protein extraction methods of fruit based on proteomics

Obtaining high- quality protein was an important for proteomic research. The extraction efficacy was significantly affected by lower protein content and lots of proteases and interfering substances in fruit. It is necessary to choose an optimum extraction method.Four protein extraction methods of fruit were reviewed in this paper and the applications and characteristics of each method were described. Among them, phenol extraction method had been widely used for obtaining a higher purity of protein, which meets to extract protein from fruit and cell wall.Another improved methods, SDS and lyolysis buffer methods, could also gain a higher yield of protein; however, the process was more complicated.TCA- acetone method was simple and convenient; however, protein could not precipitate and disintegrate completely, the method was only used for extracting peel protein.