Pretreatment and purification of poly-γ-glutamic acid from fermentation broth

HE Yang-yang; ZENG Wei; WANG Qing-long; CHEN Gui-guang; LIANG Zhi-qun

doi:10.13386/j.issn1002-0306.2014.06.009

HE Yang-yang, ZENG Wei, WANG Qing-long, CHEN Gui-guang, LIANG Zhi-qun. Pretreatment and purification of poly-γ-glutamic acid from fermentation broth[J]. Science and Technology of Food Industry, 2014, (06): 156-160. DOI: 10.13386/j.issn1002-0306.2014.06.009

Citation:

Pretreatment and purification of poly-γ-glutamic acid from fermentation broth

Abstract

Abstract

By using alcohol precipitation and salting out to extract γ-PGA. The best dilution multiples was 2 times, the dosage of diatomaceous earth and powder activated carbon (granular activated carbon) , respectively, 10g/L and 10g/L (15g/L) , the best pH value was 3.0 for suction filter under the room temperature. Using 5%NaCl and double volume of ethanol for extraction of γ-PGA, by frozen vacuum drying of γ-PGA, the sample was white and granular, extraction efficiency achieved 95.21%, purity was 96.89%, using this method, the production input had reduced and the dosage of ethanol also highly reduced.

FullText(HTML)

References (15)

Relative Articles

Supplements (0)

Cited By

Science and Technology of Food Industry

Pretreatment and purification of poly-γ-glutamic acid from fermentation broth

Abstract

Catalog

Export File

Citation

Format

Content