Study on detection of volatile components of chilled pork inoculated by different dominating spoilage bacteria

In order to study the spoilage characteristics of dominant spoilage bacteria, Pseudomonas koreensis, Clostridium fusiformis and Brochothrix thermosphacta were respectively inoculated into sterilized pork at the storage temperature of 4℃.Volatile compounds of pork with different storage time were analyzed by headspace solid micro extraction (HS-SPME) . Pork with storage time of 48, 96, 144h and dominant spoilage bacteria had a significant difference in volatile composition. As the time of cold-storage went on, the content of alkanes rose markedly, pork inoculated with Clostridium fusiformis and Brochothrix thermosphacta provided significantly higher levels of 3-hydroxy-2-Butanone and 3-methyl-Butanol, respectively. Contents of sulfhydryl methane and 3-sulfhydryl propylene were actually low. These results showed that at 0.05 significance level, there was significant difference in total volatile substance between pork inoculated by bacteria and control sample.