Optimization of extraction of protein from base of Flammulina velutipes using response surface methodology

In this study, extraction of proteins from base of Flammulina velutipes was investigated. Materials were crushed into 100 mesh powders, and 0.14mol/L NaCl buffer was used to extract for twice. Based on single factor experiments, solid-liquid ratio, extraction temperature, extraction time, and extraction pH were optimized using Box-Behnken design and response surface methodology for achieving maximum extraction rate of proteins. Factor contribution rate obtained from the F-test were as follows:solid-liquid ratio >extraction pH >extraction time >extraction temperature. The optimum extraction parameters obtained from response surface analysis were as follows:solid-liquid ratio 1 ∶16g/mL, extraction temperature 30℃, extraction time 30min, and extraction pH10. Under the optimized conditions, the predicated extraction rate of proteins was 64.75%, meanwhile the rate of proteins was 64.91% in the validated experiment with the relative deviation value of 0.25%.