Isolation and structural identification of alkaloids of papaya leaves

WANG Xiu-yi; HU Chang-ying; YU Bing; OU Shi-yi; WANG Zhi-wei

doi:10.13386/j.issn1002-0306.2014.06.073

WANG Xiu-yi, HU Chang-ying, YU Bing, OU Shi-yi, WANG Zhi-wei. Isolation and structural identification of alkaloids of papaya leaves[J]. Science and Technology of Food Industry, 2014, (06): 129-131. DOI: 10.13386/j.issn1002-0306.2014.06.073

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Isolation and structural identification of alkaloids of papaya leaves

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Abstract

Alkaloids were extracted five times from the dry papaya leaves by the solution of 90% ethanol contains1% HCl. And then, the extract was concentrated, filtered, and degreased by petroleum ether. The acidity was adjusted to pH8.0⁹.0 using concentrate NH3·H2O solution, and then extracted with excess chloroform. After purification of Silica gel column chromatography, alkaloids structural identification was performed with spectral methods (IR、MS、NMR) . The carpaine was isolated and identified.

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Isolation and structural identification of alkaloids of papaya leaves

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