Identification and controlling of producing membrane strains isolated from bagged deterioration vinegar

8 strains were isolated from the vinegar which formed cellulose membrane in shelf life.Among them, strain D23 and strain D24 was identified by 16 S r DNA combined with morphological features. And then, the influence acidity, heat sterilization conditions and preservatives exerted upon stain D23 and strain D24 were studied. The results showed that, strain D23 was identified as Gluconacetobacter oboediens and D23 was identified as Acetobacter pasteurianus.when the vinegar acidity was more than 3.5 g /100 m L, strain D23 and strain D24 could not grow or produce membrane with 2% inoculum.The thermal death condition of strain D23 and strain D24 was 60℃10min.The membrane producing strains could be well suppressed by all of the three preservatives ( ethylparaben, potassium sorbate, sodium benzoate) .