Optimization of cellulase- assisted extraction of sugarbeet boot pomace crude polysaccharides, compositions and antibacterial activity

In order to study the different parameter of crude polysaccharides from sugarbeet boot pomace, the best extracting process was ensured.Based on one- factor- at- a- time experiments, response surface methodology with four- factors- three- levels was used to optimize the cellulase- assisted extraction of crude polysaccharides from sugarbeet boot pomace ( SCPs) . The physicalchemical compositions, monosaccharide compositions and antibacterial activity of SCPs were investigated. The optimal extraction conditions was found as 30 ∶ 1 of distilled water-to-sugarbeet boot, hydrolysis time 40 min, hydrolysis temperature 52℃, p H4.5, and cellulase amount 4677 U / g.Under these conditions, the extraction yield of SCPs was 3.93%. The antibacterial assay showed that these polysaccharides had different antibacterial activity against Staphyloccocus aureus, Bacillus subtilis and Escherichia coli, and the corresponding minimum inhibitory concentrations ( MICs) were 12.5, 25 and 25 mg / m L, respectively.