Optimization of enzymatic hydrolysis conditions for casein by response surface methodology

Trypsin, pepsin, papain, alcalase and neutrase were used for casein hydrolysis in this study. Pepsin was the best enzyme for the progress from the degree of hydrolysis ( DH) determination. In single factor experiment, hydrolysis temperature, p H, enzyme concentration and time was selected to detect the better conditions for pepsin hydrolysis with casein.Based on the results, by employing response surface methodology ( RSM) and building the mathematical model for hydrolysis conditions, the optimal hydrolysis conditions were obtained as: p H1.3, enzyme concentration 1.75 mg / m L and temperature 57℃ through the regression analysis and model evaluation. After the hydrolysis for 6h under the above conditions, the DH could reach 9.45%.