Study on aqueous two- phase extraction of protease from Monopterus albus

LIU Kun; LI Deng-chao; CHEN Hui

doi:10.13386/j.issn1002-0306.2014.23.040

LIU Kun, LI Deng-chao, CHEN Hui. Study on aqueous two- phase extraction of protease from Monopterus albus[J]. Science and Technology of Food Industry, 2014, (23): 232-234. DOI: 10.13386/j.issn1002-0306.2014.23.040

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Study on aqueous two- phase extraction of protease from Monopterus albus

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Abstract

Protease was extracted and purified by the polyethylene glycol ( PEG) - salt aqueous two- phase systems from Monopterus albus. Effects of different kinds and concentrations of phasing salts, different molecular weight and concentrations of phasing PEG, p H on extraction of the protease were studied. The results showed that the optimum conditions for extraction and purification of the protease were 25% PEG1000, 20% ( NH4) 2SO4and p H7.0.Under the optimum extraction conditions, recovery and purification fold of the protease in top phase were 90.5%and 4.40, respectively.

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Study on aqueous two- phase extraction of protease from Monopterus albus

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