Optimization of the detection of the fluorescent quantitative PCR method of the meat quality gene MyHC in BaMei sheep

ZHANG Jing; GUO Yue-ying; REN Ting; CHENG Hai-xing; WANG Le; JIN Ye

doi:10.13386/j.issn1002-0306.2015.06.002

ZHANG Jing, GUO Yue-ying, REN Ting, CHENG Hai-xing, WANG Le, JIN Ye. Optimization of the detection of the fluorescent quantitative PCR method of the meat quality gene MyHC in BaMei sheep[J]. Science and Technology of Food Industry, 2015, (06): 53-57. DOI: 10.13386/j.issn1002-0306.2015.06.002

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Optimization of the detection of the fluorescent quantitative PCR method of the meat quality gene MyHC in BaMei sheep

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Abstract

The optimizing reaction condition of MyHC was found by real-time PCR,and 18S rRNA was housekeeping gene. The result showed that these primers could be used in the searching MyHC gene in BaMei sheep.Besides,when the PCR annealing temperature was 57℃,the peak of PCR melting curve was single,the cycle threshold was at the same level and the efficiency of PCR was high. So this method could be used in searching mRNA expression of the meat quality gene MyHC in BaMei sheep.

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Optimization of the detection of the fluorescent quantitative PCR method of the meat quality gene MyHC in BaMei sheep

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