Isolation,identification and drug susceptibility experiment of Salmonella from duck eggs in some parts of Sichuan

In order to understand the Salmonella spp. contamination and drug resistance from duck eggs with different rearing methods in some parts of Sichuan,the experiment detected Salmonella in eggshell and its contents with the national standard GB 4789.4-2010 detection method after collecting 100 duck eggs from intensive farms(50) and farmers backyard(50),while doing serotype identification and drug susceptibility testing. The results showed that the contamination rates of free-range egg shell were 28% and contents were30%. Meanwhile,the contamination rates of intensive farming eggshell were 24% and with no Salmonella in the contents of intensive farming egg. Serum agglutination test detected out of 8 serotypes,S.typhimurium(34.1%)was most prevalent. Besides,S.enteritidis(22%) and S.anatum(14.6%) were higher than other strains. The 41 isolated Salmonella had high drug resistance to Penicillin(100%),Ampicillin(87.80%),Amoxicillin(75.61%),Tetracycline(73.17%),showed moderate sensitivity to streptomycin and suggested that the Salmonella had different degrees of resistance to the rest of the antibiotics. These findings indicated that the Salmonella from duck eggs contamination and drug resistance had become more serious in Sichuan region, therefore, the epidemiological surveillance of salmonella should be strengthened and the use of antibiotics should be regulated,too.