Screening and identification of antagonistic strain against the main pathogens of tobacco

ZOU Fang-yun; CHEN Xiao-ming; HOU Jun; HUANG Chong-jun; WU Shou-ming; DING Ting; QI Yong-xia; LI Zhang-hai

doi:10.13386/j.issn1002-0306.2015.09.030

ZOU Fang-yun, CHEN Xiao-ming, HOU Jun, HUANG Chong-jun, WU Shou-ming, DING Ting, QI Yong-xia, LI Zhang-hai. Screening and identification of antagonistic strain against the main pathogens of tobacco[J]. Science and Technology of Food Industry, 2015, (09): 175-178. DOI: 10.13386/j.issn1002-0306.2015.09.030

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Screening and identification of antagonistic strain against the main pathogens of tobacco

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By dilution method,10 strains were isolated from Zunyi tobacco rhizosphere soil and self- made bio organic fertilizer,which had antagonistic activity against the main pathogens of tobacco. Dual culture method was used to study the antagonistic effect of the strains on pathogens mentioned- above. The results showed that the growth inhibition rate of antagonistic bacteria Y2,Y3,Y4 and Y6 were very significant; the relative inhibitory rate of Y4 against Pseudomonas solanacearum was 400%. The antagonistic bacterium Y4 was primarily identified to be Cupriavidus necator through 16 S r DNA sequence analysis.

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Science and Technology of Food Industry

Screening and identification of antagonistic strain against the main pathogens of tobacco

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