Apoptosis inducing effects of crude polyphenols of Insect tea made by Kuding tea leaves in Hep G2 human hepatoma cells

Tea polyphenols are functional substances present in tea. Insect tea as a traditional drink also contains these compounds,the anticancer effects of these polyphenols need to be scientific researched. In this study,cancer cells apoptosis inducing effects of crude polyphenols of Insect tea made by Kuding tea leaves( CPKMI)were determined.The in vitro cancer cells growth inhibitory effects of CPKMI were checked by MTT assay,the apoptosis inducing effects of CPKMI were further tested by RT- PCR assay. After 25,50 and 100μg / m L of CPKMI treatment for 48 h,cell proliferation of Hep G2 human liver cancer cells were inhibited,and the 100μg / m L of CPKMI showed the highest inhibitory rate at 72.8%.CPKMI also significantly induced apoptosis in Hep G2 cancer cells( p <0.05) by upregulating caspase- 3,caspase- 9,p53,p21,E2F1,p73 and down regulating HIAP- 1,HIAP- 2 mRNA expressions.CPKMI thus present strong cancer cells apoptosis inducing effects in vitro.