Effect of germination on phenolics and antioxidant activity of proso millet

The influence of germination on antioxidant activity,phenolics content and form of proso millet were studied. The changes of phenolics,flavonoids and antioxidant properties(DPPH and ABTS,FRAP,ORAC) of surface sterilized proso millet seeds during germination were determined in artificial climate box for 6 d at 25 ℃and RH 95%. The result showed that the contents of phenolics,flavonoids and antioxidant properties increase obviously from 2 d. Germination after 6 d,the total content of phenolics and flavonoids of NM8 increase 5.9 and1.9 times respectively,and 4.6 and 1.9 times for XN10-04,meanwhile,its antioxidant capacity increase significantly(p <0.05). In addition,total content of phenolics and flavonoids and antioxidant capacity of the sprouts were significantly higher( p < 0. 05) than that of seeds after 4 d. Correlation coefficients between total phenolics content,total flavonoids content and antioxidant activities were positively high,the highest correlation coefficient was found between total flavonoids content and FRAP(r=0.985). This study showed that germination was an effective method to increase the content of phenolic compounds and antioxidant activity of proso millet.