Screening and identification of strains for producing γ- aminobutyric acid

In order to obtain the safe strains for producing γ- aminobutyric acid,the directed screening for those strains were selected from pickled cabbage juice and yogurt. The preliminary analysis of the fermentation liquor was processed by thin layer chromatography( TLC),and then the 16 S r DNA gene sequencing and Gen Bank comparing were carried for the taxonomy identification. Finally,the high performance liquid chromatography( HPLC) was used for quantitative analysis of γ-aminobutyric acid productivity.Six γ-aminobutyric acid producing strains were screened from the nature samples.The 16 S r DNA gene sequencing and Gen Bank comparing results showed the six strains belonged to short lactic acid bacteria. The productive yield of strains were among 200 ~500 μg / m L,while the maximum yield was 493.3 μg / m L. TLC method can rapidly qualitative detect whether theγ-aminobutyric acid were contained in cultures,and the optimized HPLC method can accurately and quantitatively detect the contents of γ- aminobutyric acid in cultures. Since the biological safety of those lactic acid bacteria,those strains have potential application.