Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR

XUE Yan-feng; HAO Li-zhuang; LIU Shu-jie

doi:10.13386/j.issn1002-0306.2016.04.020

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Article Navigation > Science and Technology of Food Industry > 2016 > (04): 147-150

XUE Yan-feng, HAO Li-zhuang, LIU Shu-jie. Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR[J]. Science and Technology of Food Industry, 2016, (04): 147-150. doi: 10.13386/j.issn1002-0306.2016.04.020

Citation:

XUE Yan-feng, HAO Li-zhuang, LIU Shu-jie. Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR[J]. Science and Technology of Food Industry, 2016, (04): 147-150. doi: 10.13386/j.issn1002-0306.2016.04.020

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Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR

doi: 10.13386/j.issn1002-0306.2016.04.020

State Key Laboratory of Cultivating Base of Plateau Grazing Animal Nutrition and Ecology of Qinghai Province,Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province,Qinghai Plateau Yak Research Center,Qinghai Academy of Animal Science and Veterinary Medicine

Received Date: 2015-05-29

Abstract

Abstract

In order to research the difference of yak meat's quality in different regions,total RNA was extracted from 4-year-old male yak 's muscle samples of Datong,Jiulong,Henan,Diqing,Hongyuan,and then c DNA was generated under the action of reverse transcription polymerase,at last,the production of reserve transcription reaction was utilized to carry out RT-PCR and detect the expression levels of FABP gene. The results were that if the yak muscle sample of Diqing was taken as the control group and the 2~(-ΔΔCt)value of it was set to 1,the fluorescence quantitative 2~(-ΔΔCt)value of Datong,Jiulong,Henan,Hongyuan yak muscles were 4.18,2.94,9.95,10.73 respectively. So that the relative expression quantity of FABP gene in yak muscles could be obtained to evaluate yak meat's quality in different regions on molecular level. The yak meat's quality of Hongyuan and Henan was the best while that of Diqing was the worst.
- FABP,
- Real-time PCR,
- expression,
- yak meat quality

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References(30)

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