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XUE Yan-feng, HAO Li-zhuang, LIU Shu-jie. Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR[J]. Science and Technology of Food Industry, 2016, (04): 147-150. doi: 10.13386/j.issn1002-0306.2016.04.020
Citation: XUE Yan-feng, HAO Li-zhuang, LIU Shu-jie. Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR[J]. Science and Technology of Food Industry, 2016, (04): 147-150. doi: 10.13386/j.issn1002-0306.2016.04.020

Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR

doi: 10.13386/j.issn1002-0306.2016.04.020
  • Received Date: 2015-05-29
  • In order to research the difference of yak meat's quality in different regions,total RNA was extracted from 4-year-old male yak 's muscle samples of Datong,Jiulong,Henan,Diqing,Hongyuan,and then c DNA was generated under the action of reverse transcription polymerase,at last,the production of reserve transcription reaction was utilized to carry out RT-PCR and detect the expression levels of FABP gene. The results were that if the yak muscle sample of Diqing was taken as the control group and the 2~(-ΔΔCt)value of it was set to 1,the fluorescence quantitative 2~(-ΔΔCt)value of Datong,Jiulong,Henan,Hongyuan yak muscles were 4.18,2.94,9.95,10.73 respectively. So that the relative expression quantity of FABP gene in yak muscles could be obtained to evaluate yak meat's quality in different regions on molecular level. The yak meat's quality of Hongyuan and Henan was the best while that of Diqing was the worst.
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  • [1]
    Zi X D,Zhong G H,Wen Y L,et al.Growth performance,carcass composition and meat quality of Jiulong yak(Bos grunniens)[J].Asian Australasian Journal of Animal Sciences,2004,17(3):410-414.
    [2]
    Yin R H,Bai W L,Wang J M,et al.Development of an assay for rapid identification of meat from yak and cattle using polymerase chain reaction technique[J].Meat Science,2009,83(1):38-44.
    [3]
    Zhang Y H,Yan P,Liang C N,et al.Composition analysis on inter-muscular fatty acid of Qinghai Datong yak[J].Agricultural Science&Technology-Hunan,2009,10(1):145-148.
    [4]
    Han L.Tenderizing technology for chilled cut yak meat by fermentation[J].Transactions of the Chinese Society of Agricultural Engineering,2003,2:041.
    [5]
    张丽,师希雄,余群力,等.甘南牦牛肉质构特性分析[J].肉类研究,2013(9):19-21.
    [6]
    张兴隆,杨永在,王长水,等.育成期营养水平对育肥皖南牛背最长肌不同部位肉品质的影响[J].肉类研究,2014(11):1-6.
    [7]
    孔祥颖,张丽,保善科,等.放养过程中补饲对青海高原牦牛产肉能力及肉品质的影响[J].中国畜牧兽医,2015,42(1):104-108.
    [8]
    黄彩霞,孙宝忠,卢凌,等.宰后成熟时间与温度对牦牛肉品质影响[J].食品工业科技,2013,34(19):57-60.
    [9]
    田园,党欣,韩玲,等.不同电刺激时间对牦牛肉在成熟过程中品质变化的影响[J].食品工业科技,2014,35(1):56-60.
    [10]
    黄彩霞,高媛,孙宝忠,等.牦牛品种品质研究进展[J].肉类研究,2013(9):30-34.
    [11]
    罗毅皓,刘书杰,吴克选.大通犊牦牛肉食用品质研究[J].食品研究与开发,2010,31(1):20-23.
    [12]
    王金浩,文生萍,饶华,等.影响牛肉品质的主效基因[J].云南农业大学学报:自然科学版,2008,23(5):693-697.
    [13]
    Lee S H,Choi Y M,Choe J H,et al.Association between polymorphisms of the heart fatty acid binding protein gene and intramuscular fat content,fatty acid composition,and meat quality in Berkshire breed[J].Meat Science,2010,86(3):794-800.
    [14]
    Mannen H.Identification and utilization of genes associated with beef qualities[J].Animal Science Journal,2011,82(1):1-7.
    [15]
    Zhao C,Tian F,Yu Y,et al.Muscle transcriptomic analyses in Angus cattle with divergent tenderness[J].Molecular Biology Reports,2012,39(4):4185-4193.
    [16]
    邹艳慧,高学文.脂肪酸结合蛋白研究进展[J].内蒙古医学杂志,2010(8):952-955.
    [17]
    Romero-Isart N,Va觢ák M.Advances in the structure and chemistry of metallothioneins[J].Journal of Inorganic Biochemistry,2002,88(3):388-396.
    [18]
    Lin Y Q,Wang G S,Feng J,et al.Comparison of enzyme activities and gene expression profiling between yak and bovine skeletal muscles[J].Livestock Science,2011,135(1):93-97.
    [19]
    郝称莉,李齐发,乔永,等.湖羊肌肉组织H-FABP和PPARY基因表达水平与肌内脂肪含量的相关研究[J].中国农业科学,2008,41(11):3776-3783.
    [20]
    Michal J J,Zhang Z W,Gaskins C T,et al.The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyux Limousin F2crosses[J].Animal Genetics,2006,37(4):400-402.
    [21]
    Jurie C,Cassar-Malek I,Bonnet M,et al.Adipocyte fatty acid binding protein and mitochondrial enzyme activities in muscle as relevant indicators of marbling in cattle[J].Journal of Animal Science,2007,85(10):2660-2669.
    [22]
    Lee S H,Van der Werf J H,Lee S H,et al.Genetic polymorphisms of the bovine fatty acid binding protein 4 gene are significantly associated with marbling and carcass weight in Hanwoo(Korean cattle)[J].Animal Genetics,2010,41(4):442-444.
    [23]
    Hoashi S,Hinenoya T,Tanaka A,et al.Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese black cattle[J].BMC Genetics,2008,9(1):84-90.
    [24]
    Chos S,Park T S,Yoon D H,et al.Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with black fat thickness in Korean native cattle[J].BMC Reports,2008,41(1):29-34.
    [25]
    Barendse W,Bunch R J,Thomas M B,et al.A splice site single nucleotide polymorphism of the fatty acid binding protein4 gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle[J].Animal Genetics,2009,40(5):770-773.
    [26]
    Narukami T,Sasazaki S,Oyama K,et al.Effect of DNA polymorphisms related to fatty acid composition in adipose tissue of Holstein cattle[J].Animal Science Journal,2011,82(3):406-411.
    [27]
    谢荣清,罗光荣,杨平贵,等.不同年龄牦牛肉肉质测试与分析[J].草食家畜,2006(4):33-36.
    [28]
    邱翔,张磊,文勇立,等.四川牦牛、黄牛主要品种肉的营养成分分析[J].食品科学,2010(15):112-116.
    [29]
    张永辉.大通牦牛肉质特性研究[D].兰州:甘肃农业大学,2009.
    [30]
    刘勇.犊牦牛肉用品质,脂肪酸及挥发性风味物质研究[D].兰州:甘肃农业大学,2010.
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