A preliminary study of inhibitory activities on angiotensin I-converting enzyme and antihypertensive effects of synthetic dipeptide

WANG Jian-guo; YAN Bing-yu; LEI Jian-yong; WU Yue; GUO Lei; XING Ke

doi:10.13386/j.issn1002-0306.2016.04.023

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WANG Jian-guo, YAN Bing-yu, LEI Jian-yong, WU Yue, GUO Lei, XING Ke. A preliminary study of inhibitory activities on angiotensin I-converting enzyme and antihypertensive effects of synthetic dipeptide[J]. Science and Technology of Food Industry, 2016, (04): 160-164. doi: 10.13386/j.issn1002-0306.2016.04.023

Citation:

WANG Jian-guo, YAN Bing-yu, LEI Jian-yong, WU Yue, GUO Lei, XING Ke. A preliminary study of inhibitory activities on angiotensin I-converting enzyme and antihypertensive effects of synthetic dipeptide[J]. Science and Technology of Food Industry, 2016, (04): 160-164. doi: 10.13386/j.issn1002-0306.2016.04.023

Citation:

WANG Jian-guo, YAN Bing-yu, LEI Jian-yong, WU Yue, GUO Lei, XING Ke. A preliminary study of inhibitory activities on angiotensin I-converting enzyme and antihypertensive effects of synthetic dipeptide[J]. Science and Technology of Food Industry, 2016, (04): 160-164. doi: 10.13386/j.issn1002-0306.2016.04.023

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A preliminary study of inhibitory activities on angiotensin I-converting enzyme and antihypertensive effects of synthetic dipeptide

doi: 10.13386/j.issn1002-0306.2016.04.023

State Key Laboratory of Biocontrol,Sun Yat-sen University
Withyou Biotech Co.,Ltd.

Received Date: 2015-07-02

Abstract

Abstract

Objective:The purpose of this work was to study the inhibitory activities on angiotensin I-converting enzyme(ACE) and the antihypertensive effects of synthetic dipeptides. Methods:The inhibitory activities on ACE of35 synthetic dipeptides were screened by using ultraviolet and visible spectrophotometry. The IC50 of dipeptides with high inhibitory activity was also measured. The antihypertensive effects of dipeptides were subsequently studied in spontaneously hypertensive rats(SHR) at 2 h and 4 h after oral administration diluted in 1 m L normal saline according to the dosages of 1,10,25,50,100 mg/kg,respectively. Results:With the exception of a few dipeptides,most of dipeptides showed ACE inhibitory activities. Among them,Cys-Ala(CA) and Cys-His(CH)were dipeptides with the highest ACE inhibitory activities,of which the ACE inhibition rates were 84.38% and 70.79%respectively. The dose response data showed the IC50 values of CA and CH were 23.22 μmol/L and 61.17 μmol/L respectively. The antihypertensive effects of CA in spontaneously hypertensive rats(SHR) were further studied. The data showed obvious antihypertensive effect of CA in SHR. The results also revealed that the antihypertensive effects after 2 h of administration was positively correlated with doses of oral administration(R2>0.8),and the overall antihypertensive effects after 4 h of administration were higher than that after 2 h of administration. Conclusion:Based on the results of determination on in vitro ACE inhibitory activities and in vivo experiments,CA was the dipeptide with high ACE inhibition rate and strong antihypertensive effect,which suggested CA could be used as functional food ingredients with potential therapeutic benefit in the prevention and treatment of hypertension.
- angiotensin I-converting enzyme(ACE),
- dipeptide,
- hypertension

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References(22)

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