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FU Rui- min, XING Wen-hui, ZHANG Hong, ZHANG Li-qin, CHEN Wu-ling. Breeding of antagonistic bacteria against Penicillium expansum and study on its inhibition mechanism[J]. Science and Technology of Food Industry, 2016, (05): 154-158. doi: 10.13386/j.issn1002-0306.2016.05.021
Citation: FU Rui- min, XING Wen-hui, ZHANG Hong, ZHANG Li-qin, CHEN Wu-ling. Breeding of antagonistic bacteria against Penicillium expansum and study on its inhibition mechanism[J]. Science and Technology of Food Industry, 2016, (05): 154-158. doi: 10.13386/j.issn1002-0306.2016.05.021

Breeding of antagonistic bacteria against Penicillium expansum and study on its inhibition mechanism

doi: 10.13386/j.issn1002-0306.2016.05.021
  • Received Date: 2015-04-27
  • To breed the antagonistic strain which can inhibit Penicillium expansum effectively,and discuss its antibacterial mechanism preliminarily.An antagonistic strain BA-16,which could inhibit Penicillium expansum,was isolated from apple surface and identified based on phenotypic,physiological,biochemical and phylogenetic( 16 S r DNA) studies.In order to enhance its antagonistic capability,the mutation of BA-16 was carried out by using low energy N+implantation.Double enzyme reaction system was used to detect the phospholipase A( PLA) activity of Penicillium expansum under the action of wild- type antagonistic strain and its mutant. The strain BA- 16 was identified as Bacillus amyloliquefaciens.After the low energy N + implantation,the mutant BA-16-8,which showed the strongest antagonist capability and stable hereditary stability was selected out. In phospholipase activity detection,mutant showed stronger inhibition against PLA activity secreted by Penicillium expansum than its wild-type strain. Moreover,as the concentration of fermentation broth increased,the inhibition effect enhanced,which indicated that it was possibly the inhibitory mechanism of antagonistic strain against Penicillium expansum. The mutant BA-16-8 will be a potential biological control agent against apple blue mold decay.
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