Issue 05
Turn off MathJax
Article Contents
MIAO Lu- huan, DU Jing- fang, BAI Feng- ling, LI Jian-rong. Progress of toxic metabolite analysis in fermented food by metabolomics[J]. Science and Technology of Food Industry, 2016, (05): 388-393. doi: 10.13386/j.issn1002-0306.2016.05.071
Citation: MIAO Lu- huan, DU Jing- fang, BAI Feng- ling, LI Jian-rong. Progress of toxic metabolite analysis in fermented food by metabolomics[J]. Science and Technology of Food Industry, 2016, (05): 388-393. doi: 10.13386/j.issn1002-0306.2016.05.071

Progress of toxic metabolite analysis in fermented food by metabolomics

doi: 10.13386/j.issn1002-0306.2016.05.071
  • Received Date: 2015-06-05
  • Some toxic metabolites were produced during the process of food fermentation,which caused safety problems to fermented food. As a new technology,metabolomics can analyze and monitor small molecule metabolites of biological metabolism in real time. Technologies of gas chromatogram- mass spectrometry( GC-MS),ion chromatography( IC),reversed- phase high performance liquid chromatography( RP- HPLC),HPLC- MS / MS and liquid chromatography- high resolution time of flight mass spectrometer( LC- ESI- TOFMS)were used to analyze toxic metabolites in food such as alcoholic beverage,wine,cheese,sausage,soybean sauce. The formation and changes of ethyl carbamate,biogenic amine and nitrite were analyzed at different fermentation period.It can provide references for increasing the safety of fermented food and promoting application in the field of fermented food.
  • loading
  • [1]
    Putri S P,Nakayama Y,Matsuda F,et al.Current metabolomics:practical applications[J].Journal of Bioscience and Bioengineering,2013,115(6):579-589.
    [2]
    Trujillo E,Davis C,Milner J.Nutrigenomics,proteomics,metabolomics,and the practice of dietetics[J].Journal of the American Dietetic Association,2006,106(3):403-413.
    [3]
    Wishart D S.Metabolomics:applications to food science and nutrition research[J].Trends in Food Science&Technology,2008,19(9):482-493.
    [4]
    Castro-Puyana M,Herrero M.Metabolomics approaches based on mass spectrometry for food safety,quality and trace ability[J].Tr AC Trends in Analytical Chemistry,2013,52:74-87.
    [5]
    Oldiges M,Lütz S,Pflug S,et al.Metabolomics:current state and evolving methodologies and tools[J].Applied Microbiology and Biotechnology,2007,76(3):495-511.
    [6]
    Fiehn O.Combining genomics,metabolome analysis,and biochemical modelling to understand metabolic networks[J].Comparative and Functional Genomics,2001,2(3):155-168.
    [7]
    孙茂成,李艾黎,霍贵成,等.乳酸菌代谢组学研究进展[J].微生物学通报,2012,39(10):1499-1505.
    [8]
    Li Z Y,Zhi H J,Zhang F S,et al.Metabolomic profiling of the antitussive and expectorant plant Tussilago farfara L.by nuclear magnetic resonance spectroscopy and multivariate data analysis[J].Journal of Pharmaceutical and Biomedical Analysis,2013,75(5):158-164.
    [9]
    Longini M,Giglio S,Perrone S,et al.Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn:a metabolomic approach[J].Clinica Chimica Acta,2015,444(15):250-256.
    [10]
    Pace R,Martinelli E M,Sardone N,et al.Metabolomic evaluation of ginsenosides distribution in Panax genus(Panax ginseng and Panax quinquefolius)using multivariate statistical analysis[J].Fitoterapia,2015,101:80-81.
    [11]
    González-Domínguez R,García-Barrera T,Vitorica J,et al.Deciphering metabolic abnormalities associated with alzheimer’s disease in the APP/PS1 mouse model using integrated metabolomic approaches[J].Biochimie,2015,110:119-128.
    [12]
    Kang H J,Yang H J,Kim M J,et al.Metabolomic analysis of meju during fermentation by ultra performance liquidchromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF MS)[J].Food Chemistry,2011,127(3):1056-1064.
    [13]
    Lee J E,Lee B J,Chung J O,et al.Metabolomic unveiling of a diverse range of green tea(Camellia sinensis)metabolites dependent on geography[J].Food Chemistry,2015,174(1):452-459.
    [14]
    Lopez-Sanchez P,Vos R C H D,Jonker H H,et al.Comprehensive metabolomics to evaluate the impact of industrial processing on the phytochemical composition of vegetable purees[J].Food Chemistry,2015,168:348-355.
    [15]
    Raterink R J,Lindenburg P W,Vreeken R J,et al.Recent developments in sample-pretreatment techniques for mass spectrometry-based metabolomics[J].Tr AC Trends in Analytical Chemistry,2014,61:157-167.
    [16]
    Lee S H,Jung J Y,Jeon C O.Effects of temperature on microbial succession and metabolite change during saeujeot fermentation[J].Food Microbiology,2014,38:16-25.
    [17]
    Lee S Y,Lee S,Lee S,et al.Primary and secondary metabolite profiling of doenjang,a fermented soybean paste during industrial processing[J].Food Chemistry,2014,165(15):157-166.
    [18]
    Xu Y J,Wang C,Ho W E,et al.Recent developments and applications of metabolomics in microbiological investigations[J].Tr AC Trends in Analytical Chemistry,2014,56:37-48.
    [19]
    Marcone M F,Wang S,Albabish W,et al.Diverse foodbased applications of nuclear magnetic resonance(NMR)technology[J].Food Research International,2013,51(2):729-747.
    [20]
    Kim H K,Choi Y H,Verpoorte R.NMR-based metabolomic analysis of plants[J].Nature Protocols,2010,5(3):536-549.
    [21]
    Papotti G,Bertelli D,Graziosi R,et al.Traditional balsamic vinegar and balsamic vinegar of Modena analyzed by nuclear magnetic resonance spectroscopy coupled with multivariate data analysis[J].LWT-Food Science and Technology,2015,60(2):1017-1024.
    [22]
    Jeong S H,Jung J Y,Lee S H,et al.Microbial succession and metabolite changes during fermentation of dongchimi,traditional Korean watery kimchi[J].International Journal of Food Microbiology,2013,164(1):46-53.
    [23]
    Antignac J P,Courant F,Pinel G,et al.Mass spectrometrybased metabolomics applied to the chemical safety of food[J].Tr AC Trends in Analytical Chemistry,2011,30(2):292-301.
    [24]
    Gu Q,David F,Lynen F,et al.Evaluation of automated sample preparation,retention time locked gas chromatographymass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species[J].Journal of Chromatography A,2011,1218(21):3247-3254.
    [25]
    Cox D G,Oh J,Keasling A,et al.The utility of metabolomics in natural product and biomarker characterization[J].Biochimica et Biophysica Acta(BBA)-General Subjects,2014,1840(12):3460-3474.
    [26]
    Issaq H J,Van Q N,Waybright T J,et al.Analytical and statistical approaches to metabolomics research[J].Journal of Separation Science,2009,32(13):2183-2199.
    [27]
    Ruben T,Kris M,Dieter D,et al.Joint GC-MS and LC-MS platforms for comprehensive plant metabolomics:repeatability and sample pre-treatment[J].Journal of Chromatography B Analytical Technologies in the Biomedical&Life Sciences,2009,877(29):3572-3580.
    [28]
    Park M K,Cho I H,Lee S,et al.Metabolite profiling of Cheonggukjang,a fermented soybean paste,during fermentation by gas chromatography-mass spectrometry and principal component analysis[J].Food Chemistry,2010,122(4):1313-1319.
    [29]
    Boucher C L,Courant F,Jeanson S,et al.First mass spectrometry metabolic fingerprinting of bacterial metabolism in a model cheese[J].Food Chemistry,2013,141(2):1032-1040.
    [30]
    Ellis D I,Brewster V L,Dunn W B,et al.Fingerprinting food:current technologies for the detection of food adulteration and contamination[J].Chemical Society Reviews,2012,41(17):5706-5727.
    [31]
    Mozzi F,Ortiz M E,Bleckwedel J,et al.Metabolomics as a tool for the comprehensive understanding of fermented and functional foods with lactic acid bacteria[J].Food Research International,2013,54(1):1152-1161.
    [32]
    Piras C,Marincola F C,Savorani F,et al.A NMR metabolomics study of the ripening process of the Fiore Sardo cheese produced with autochthonous adjunct cultures[J].Food Chemistry,2013,141(3):2137-2147.
    [33]
    成黎.传统发酵食品营养保健功能与质量安全评价[J].食品科学,2012,33(1):280-284.
    [34]
    LARC.Alcoholic beverage consumption and ethyl carbamate(urethane)[M].Geneva:WHO,2007.
    [35]
    Júnior J C B,Mendona R C S,Pereira J A M,et al.Ethylcarbamate determination by gas chromatography-mass spectrometry at different stages of production of a traditional Brazilian spirit[J].Food Chemistry,2011,129(4):1383-1387.
    [36]
    Wu P,Pan X,Wang L,et al.A survey of ethyl carbamate in fermented foods and beverages from Zhejiang,China[J].Food Control,2012,23(1):286-288.
    [37]
    Weber J V,Sharypov V I.Ethyl carbamate in foods and beverages:a review[J].Environmental Chemistry Letters,2009,7(3):233-247.
    [38]
    Park S K,Kim C T,Lee J W,et al.Analysis of ethyl carbamate in Korean soy sauce using high-performance liquid chromatography with fluorescence detection or tandem mass spectrometry and gas chromatography with mass spectrometry[J].Food Control,2007,18(8):975-982.
    [39]
    Wu P,Cai C,Shen X,et al.Formation of ethyl carbamate and changes during fermentation and storage of yellow rice wine[J].Food Chemistry,2014,152(1):108-112.
    [40]
    Lim H S,Lee K G.Development and validation of analytical methods for ethyl carbamate in various fermented foods[J].Food Chemistry,2011,126(3):1373-1379.
    [41]
    Lea J M,Pereira V,Pereira A C,et al.Rapid and sensitive methodology for determination of ethyl carbamate in fortified winesusing microextraction by packed sorbent and gas chromatography with mass spectrometric detection[J].Analytica Chimica Acta,2014,811(6):29-35.
    [42]
    Chin-Chen M L,Carda-Broch S,Peris-Vicente J,et al.Evaluation of biogenic amines in fish sauce by derivatization with3,5-dinitrobenzoyl chloride and micellar liquid chromatography[J].Journal of Food Composition and Analysis,2013,29(1):32-36.
    [43]
    Shalaby A R.Significance of biogenic amines to food safety and human health[J].Food Research International,1996,29(7):675-690.
    [44]
    Shukla S,Park H K,Lee J S,et al.Reduction of biogenic amines and aflatoxins in Doenjang samples fermented with various Meju as starter cultures[J].Food Control,2014,42(2):181-187.
    [45]
    Ruiz-Capillas C,Jiménez-Colmenero F.Biogenic amines in meat and meat products[J].Critical Reviews in Food Science and Nutrition,2005,44(7-8):489-599.
    [46]
    Gianotti V,Chiuminatto U,Mazzucco E,et al.A new hydrophilic interaction liquid chromatography tandem mass spectrometry method for the simultaneous determination of seven biogenic amines in cheese[J].Journal of Chromatography A,2008,1185(2):296-300.
    [47]
    Mey E D,Klerck K D,Maere H D,et al.The occurrence of N-nitrosamines,residual nitrite and biogenic amines in commercial dry fermented sausages and evaluation of their occasional relation[J].Meat Science,2014,96(2):821-828.
    [48]
    Arbulu M,Sampedro M C,Gómez-Caballero A,et al.Untargeted metabolomic analysis using liquid chromatography quadrupole time-of-flight mass spectrometry for non-volatile profiling of wines[J].Analytica Chimica Acta,2015,858(9):32-41.
    [49]
    Martuscelli M,Arfelli G,Manetta A C,et al.Biogenic amines content as a measure of the quality of wines of Abruzzo(Italy)[J].Food Chemistry,2013,140(3):590-597.
    [50]
    Wang Y,Li F,Zhuang H,et al.Effects of plant polyphenols and a-tocopherol on lipid oxidation,residual nitrites,biogenic amines,and N-nitrosamines formation during ripening and storage of dry-cured bacon[J].LWT-Food Science and Technology,2014,60(1):199-206.
    [51]
    GB 2762-2012,食品中污染物限量[S].北京:中国标准出版社,2012.
    [52]
    Iammarino M,Taranto A D,Cristino M.Endogenous levels of nitrites and nitrates in wide consumption foodstuffs:Results of five years of official controls and monitoring[J].Food Chemistry,2013,140(4):763-771.
    [53]
    刘广福,王硕,朱兴旺,等.接种发酵和自然发酵酸菜的亚硝酸盐含量对比分析[J].中国酿造,2013,32(7):74-76.
    [54]
    Akyüz M,Ata爦.Determination of low level nitrite and nitrate in biological,food and environmental samples by gas chromatography-mass spectrometry and liquid chromatography with fluorescence detection[J].Talanta,2009,79(3):900-904.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (84) PDF downloads(756) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return