Extraction and its antioxidant,immune activities of total flavonoids from Elsholtzia bodinieri Vaniot

The extract conditions of total flavonoids from Elsholtzia bodinieri Vaniot were optimized by designing orthogonal test.Its antioxidant activity was assessed thorugh the superoxide anion radical and hydrogen peroxide scavenging ratio.Its immune activity was researched by carbon clearance test.The optimal extraction conditions were determined as follow: the extraction temperature was 60 ℃,the reflux time was 3 h,the liquid- material ratio was 1∶ 20( g / m L).At the range of concentration of 50 ~ 200 μg / m L,the scavenging ratio for O₂^-· and H₂O₂ was26.36% ~73.94% and 50.56% ~ 77.39%,and IC₅₀ was 108.07 μg / m L and 46.53 μg / m L,respectively.The carbon clearance index K and phagocytic ratio α were increased significant in the high- dose group and middle- dose group.Thus,total flavonoids from Elsholtzia bodinieri Vaniot possessed good antioxidant and immune activities.