Determination of biogenic amines in the fermentation process of grass carp surimi by a high- performance liquid chromatography method

The effects of one group mixed starter cultures,combined with Bacillus natto and Lactobacillus pentosus,and a batch without starter as control on biogenic amines accumulation in the grass carp surimi during fermentation were investigated.Methods of simultaneous determination of six biogenic amines( tryptamine,putrescine,cadaverine,histamine,tyramine,spermine) by high- performance liquid chromatography were developed.The method had a good resolution in 30 minutes and good linearity( R2> 0.999),the recovery rate was between 97.35% ~103.00%,and the RSD of precision,repeatability were all lower than 10%.Fermentation inoculated with mixed starter cultures can inhibited the growth of contaminant microoroganisms,such as Staphylococcus aureus,Enterobacteriaceae and Pseudomonas, and suppressed the accumlation of putrescine,cadaverine,histamine,tyramine.In order to improve hygienic quality of fermented surimi,Bacillus natto,Lactobacillus pentosus were added to activate the function of negative- decarboxylase which can prevent biogenic amine formation,inhibited the spoilage bacteria and pathogens.