Protective effects of wheat germ antioxidant peptide on proliferation of vascular smooth muscle cells exposed to high glucose

Objective: To evaluate the protective effects of wheat germ antioxidant peptide( AOP) on proliferation of vascular smooth muscle cells( VSMCs) exposed to high glucose. Methods: VSMCs were randomly divided into 3groups,normal control group( 5.5 mmol / L glucose),model control group( 25 mmol / L glucose) and treatment groups( 25 mmol / L glucose in different concentration of AOP).MTT( 3-( 4,5- dimethylthiazol-2- yl)-2,5- diphenyl tetrazolium bromide) assays were used to measure VSMCs proliferation,cell cycle distribution was analyzed by flow cytometry. The activities of total antioxidation capacity( T- AOC),glutathione peroxidase( GSH- Px),superoxide dismutase( SOD) and malondialdehyde( MDA) were measured by enzyme- linked immunosorbent assay.Results: VSMCs proliferation that was enhanced by high glucose was effectively suppressed by wheat germ AOP( p < 0.01),and the cell cycle G1/ S phase transition was also blocked. In addition,antioxidative enzyme( T-AOC,GSH- Px and SOD) activities in VSMCs exposed to high glucose were significantly increased in the presence of AOP( p < 0.01),whereas the MDA level was decreased( p < 0.01).Conclusion: These results indicated that wheat germ AOP exhibited significant protective effects on proliferation of VSMCs exposed to high glucose.