Process research of taurine extraction from oyster(Crassostrea hongkongensis) by high hydrostatic pressure

In this study,High Hydrostatic Pressure( HHP) was used to extract taurine from oyster,then the taurine content were determined by High Performance Liquid Chromatography( HPLC),and the results of HHP and Hot Water Extraction( HWE) were compared.Results of the single factor experiments showed that pressure,pressureholding time,temperature and solid- liquid ratio had significant influence on the extraction of taurine.Orthogonal test based on the results of the single factor experiments showed that the optimum conditions were pressure of300 MPa,pressure- holding time of 25 min,temperature of 45 ℃,Solid- liquid ratio of 1∶ 5; and the content of taurine was 22.96 mg / g under the optimum conditions which was higher than the HWE extraction( 17.07 mg / g).