Study on optimization of fermentation conditions for preparing antioxidant peptides of Pinctada fucata by recombinant E.coil

In order to improve the efficiency of preparing antioxidant peptides of Pinctada fucata by recombinant E.coil( DE3-pEGX6p-1-PFMAP),fermentation factors including induction temperature,induction time,concentration of IPTG,culture medium p H,shaker speed and inoculum size were optimized respectively in shake flask.Furthermore,the orthogonal experiment for the pilot-scale was optimized. Results showed that the best fermentation conditions of both shaking flask and orthogonal experiment,including induction temperature,induction time,concentration of IPTG,culture medium pH,shaker speed and inoculum size were 30 ℃,4 h,0.2 mmol / L,p H7,220 r / min,5%( v / v) respectively.And the process in this study was easy to operate and take a shorter time,which help further industrial application for preparing the antioxidant peptides of Pinctada fucata by recombinant E.coil.