Cloning and bioinformatic analyzing of Unigene100791 base on safflower transcriptome sequence

Objective: To clone the Unigene 100791,study its expression,its characteristics was investigated using bioinformatics methods in order to laid a theoretical basis for subsequent metabolidm and improve the yield of safflower oil,which can meet the market demand of food industry. Methods: The fragment of Unigene 100791,related Dof transcription factors,was obtained by screening from safflower transcriptome data. Total RNA was extracted by using RNAiso plus reagent,using RT-PCR method to obtain the c DNA in safflower seeds. The full length sequence of Unigene 100791 was obtained with 3'Race method.The full length sequence of Unigene 100791 was analyzed using the bioinformatics methods,and the phylogenetic treewas constructed to study the homology,physicochemical property and structure characteristics in order to predict its biological function.Results: The 1274 bp Unigene100791 sequence in safflower was successfully cloned,which had a 981 bp ORF,encoding 326 amino acids.Its molecular weight was 35026.5 u,and the theoretical isoelectric point was 9.5,positively charged residues of 27,negatively charged residues of 18. The sequence contained a typical tailing signal poly( A). The encoding product of Unigene100791 in safflower had a closer genetic relationship with tobacco Dof2.4-1,belonging to the superfamily Dof,and this protein existed a signal peptide but had no transmembrane structure. Conclusion: The Unigene100791 was successfully cloned in safflower and used the bioinformatics approaches to analyze the encoded protein for Unigene100791.