Analysis of salbutamol sulfate and ractopamine hydrochloride with ultrasonic assisted in-situ ionic liquid dispersive liquid phase microextraction and HPLC

Objective: To bring forth a method that analyzes salbutamol sulfate and ractopamine hydrochloride with Ultrasonic assisted in-situ ionic liquid dispersive liquid phase microextraction( UAIS-IL-DLPME) and HPLC.Method: the environmentally-friendly ionic liquidC_6MIMBF_4,ion exchange agent Li NTF_2 and in-situ formationC_6MIMNTF_2were exploited to extract and concentrate the targeted compounds RAC and SAL in the newly generated hydrophobic ionic liquid for the sake of extraction,separation and concentration. Result: The optimal extraction conditions were determined as follows: extraction solvent for 20 μLC_6MIMBF_4,ion exchanger for Li NTF_2,salt concentration for 4%,dispersant for 30 μL tetrahydrofuran,ultrasonic oscillation time of 35 min,the centrifugal speed and time respectively was 12000 r / min and 10 min,SAL and RAC preconcentration factor was42.3 and 39.5,the linear range of 10 ng / m L to 100 ng / mL. Conclusion: the analysis based on the combination of UAIS-IL-DLPM-E and HPLC has helped to concentrate,purify and quickly detect the targeted compounds RAC and SAL in feed,animal innards and muscle samples.