Effect of fructose treatment on flavonoids content and the related enzymes in Sedum aizoon L.

In this paper, the effects of water culture temperature ( 20, 25, 28 ℃) , photoperiod ( 8 h/16 h, 12 h/12 h, 16 h/8 h) and fructose treatment ( 0, 10, 20, 30, 40 mmol/L) on flavonoid content and anabolism related enzyme activity in Sedum Aizoon L.were investigated.The results indicated that optimal water culture conditions was 25 ℃, 16 h/8 h.30 mmol/L fructose treatment could effectively improve the content of flavonoids in Sedum aizoon L., which the treatment group was 1.37 mg/g higher than the control group ( fresh weight) . Compared with control group, fructose treatment could delay the decrease of the activities of phenylalanine ammonia lyase ( PAL) , polyphenol oxidase ( PPO) , chalcone synthase ( CHS) and chalcone isomerase ( CHI) , and inhibit the peroxidase ( POD) activity. It suggested that fructose treatment regulated the enzymes activities of flavonoids metabolism and promoted the accumulation of flavonoids during culturing.These results laid a theoretical basis for enhancement of the bioactive compounds contents and nutritional values of Sedum aizoon L.