Science and Technology of Food Industry

ISSN 1002-0306
CN   11-1759/TS
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HUANG Yan-na, LIU Zhen-min, YOU Chun-ping. Over-expression of D-lactate dehydrogenase isoenzyme genes from Lactobacillus delbrueckii spp.bulgaricus in Escherichia coli[J]. Science and Technology of Food Industry, 2017, (05): 159-163. DOI: 10.13386/j.issn1002-0306.2017.05.021
Citation: HUANG Yan-na, LIU Zhen-min, YOU Chun-ping. Over-expression of D-lactate dehydrogenase isoenzyme genes from Lactobacillus delbrueckii spp.bulgaricus in Escherichia coli[J]. Science and Technology of Food Industry, 2017, (05): 159-163. DOI: 10.13386/j.issn1002-0306.2017.05.021
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Over-expression of D-lactate dehydrogenase isoenzyme genes from Lactobacillus delbrueckii spp.bulgaricus in Escherichia coli

    Abstract
  • Five D-LDH isoenzyme genes ( ldb0101, ldb0813, ldb1010, ldb1147 and ldb2021) from L. bulgaricus ATCC11842 were separated and over-expressed in Escherichia coli JM109 using p UC18 as the expression plasmid. The shake-flask fermentation of recombinant E.coli indicated that the D-LDH enzymes Ldb0101 and Ldb1010 played more important roles in D-lactic acid biosynthesis in L.bulgaricus.
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