Survey of the pollution of Vibrio parahaemolyticus from three shellfish products in Zhangzhou City

To analyze the carrying status of Vibrio parahaemolyticus of shellfish products in Zhangzhou City and provide reference for the food safety problem of shellfish. The fresh sample were collected by sterile operation from 8 markets in Zhangzhou City.The suspected colony was obtained by culturing on TCBS plates and detected using PCR amplification of a specific tlh gene. The positive strain was verified using biochemical test and Gram staining. 53 Venerupis philippinarum, 50 Meretrix and 42 Scalprum samples were collected from 8 main markets, 37 Venerupis philippinarum, 45 Meretrix and 36 Scalprum suspected colony were screening with TCBS selective medium.The separation rate were 69.8%, 90.0% and 85.7% for Venerupis philippinarum, Meretrix and Scalprum, respectively. PCR positive rate were 35.8% ( 19/53) , 30.0% ( 15/50) and54.8% ( 23/42) . These positive strain were confirmed by Gram staining and biochemical test. The shellfish products in Zhangzhou City are polluted by Vibrio parahaemolyticus, seafood should be strictly disposed, to avoid cross contamination between different types of aquatic products.