Changes of bacterial flora composition and identification of the dominant spoilage bacteria in turbot under simulated of room temperature logistics

Turbots stored under simulated of room temperature logistics were studied in this essay.Selective medium method and16 S r DNA sequence analysis technology were used to study the change of bacterial flora composition in turbot during simulated of room temperature logistics. The results showed that the micro flora composition of turbot were different before and after storage.The 16 S r DNA analysis, NCBI database and phylogenetic tree showed that the micro flora composition were Ameromonas sp.、Enterobacteriaceae sp.、Rhodobacteraceae sp.、Photo bacterium sp.、Gammaproteobacteria sp.、Shewanella sp.、Bacteroidales sp.and Nitriliruptoraceae sp.. The dominant spoilage bacteria of turbot stored in simulated of room temperature logistics were Aeromonas sp.、Citrobacter sp.and Hafnia sp..