Protection and mechanism of extractum phyllanthus emblica on acute alcohol-induced liver injury in mice

To study the preventive protection effects and mechanisms of extractum phyllanthus emblica ( EPE) on acute alcoholic liver injury in mice. 60 ICR male mice were randomly divided into 6 groups, including blank group, model group, medicine contrast group ( Metadoxine Capsules, 200 mg/kg·d) , EPE group ( 80、160、400 mg/kg·d) . Medication groups were given with EPE or Metadoxine Capsules respectively by intragastric administration for 30 days.Then, acute alcoholic liver injury model was established by intragastric administration of 56% alcohol ( 13 mg/kg) to the medication and model groups 1 h later after the last administration.12 h later, the activities of serum ALT, AST, TG, TNF-α, LI-6, IL-10, the content of hepatic MDA, SOD, GSH-Px, ADH, the mRNA levels of FAS, ADRP, CYP2E1, PPARα and Caspase3 in liver were measured. HE staining was performed for observing pathological changes of the liver tissues.The results showed that EPE can significantly reduce the level of serum ALT, AST, TG and the liver pathological damage. EPE can significantly reduce the sober time by improving the activities of hepatic alcohol metabolism enzyme ADH, CAT, meanwhile EPE reduce CYP2E1 mRNA expression. EPEsignificantly suppress the synthesis and transport of fatty acid by inhibiting the expression of FAS and ADRP.EPE significantly increased the activities of SOD and GSH-Px, lower MDA concentration to protect liver injury by antioxidant activity. EPE significantly reduced the content of inflammatory factor TNF-α and IL-6 to relieve liver inflammatory lesions, but EPE had no significant effects on the IL-10 content. EPE significantly reduced the level of liver cell apoptosis by down regulating the expression of Caspase3. EPE significantly relieved liver oxidative damage and inflammatory lesions by up regulating the expression of PPARα. Conclusion: EPE by ethanol metabolic enzyme activity regulation, lipid metabolic control, resistance to oxidation damage, anti-inflammatory and anti cell apoptosis can protect acute alcoholic liver injury in mice. EPE has the prospect of development to avoid hangover and be liver protection health food.