Research on the adsorption of the modified oxalate decarboxylase on calcium oxalate

In order to improve the applicability and adsorption property of oxalate decarboxylase ( OXDC) on calcium oxalate.EDTAD was used to modify the OXDC, modified site was determined by UPLC-MS, and the adsorption of the enzymes on calcium oxalate ( Ca Ox) were studied and compared. The kinetics and thermodynamics adsorption models were used to fit and analysis the experimental data. Results showed that EDTAD was successfully grafted onto OXDC covalently as an absorption group, the modified sites were located in the α-NH2 of the peptide N side, and the adsorption property of the modified enzyme ( EDTAD-OXDC) on calcium oxalate was 53.37% higher than that of OXDC in initial enzyme concentration of 5 mg/m L, p H7.0, temperature of 37 ℃. Conclusion: which inclicated the stability and adsorption properties of DXDC was improved by grafting with EDTAD.