Study on the extraction and antioxidant activity of total phenolic compounds from Dictyophora indusiata

The contents of polyphenols from Dictyophora indusiata were determined by the Folin-Ciocalteu method in the paper.The effects of optimal alcohol concentration, liquid-solid ratio, extraction time and extraction temperature yield of D.indusiata were investigated, and the extraction technology was optimized by single-factor experiments and orthogonal design.The antioxidant activity of D.indusiata phenols was also studied.The results showed that the optimal extraction conditions were found that the optimal alcohol concentration, solid-liquid ratio, extraction time and extraction temperature were 40%, 1∶ 40 g/m L, 80 ℃ and 5 h respectively.Under these conditions, the largest extraction quantity of total phenols could reach ( 8.18± 0.52) mg/g.The DPPH radical scavenging ratio and ·OH radicals of the polyphenol extracts reached 84.28% ± 1.15% and76.49% ± 1.14% respectively when the extract concentration was 20 mg/m L.