Optimization of fermentative conditions for high yield blue pigment from Streptomyces sp.YLA0 using response surface methodology

Streptomyces sp. YLA0 was used as the experimental material to optimize the culture condition of high yield blue pigment.Based on the single factor experiment, C/N ratio, p H and culture time as three single factors were selected and optimized by Box-Behnken response surface design.The results showed that the optimal culture conditions of Streptomycin sp.YLA0 were as follows: C/N ratio was 14∶ 1, p H7.63, culture time was 169.69 h, and the yield was 11.67 mg/100 m L.Under this condition, the actual value was 97.04% of the optimized value, and the results showed that the model was reliable and could be used to optimize the culture condition of Streptomyces sp.YLA0.