Optimization of enzymolysis conditions for degradation of agarose by cellulase

In this paper, the conditions of cellulose enzymatic degradation of agarose were studied with the yield of reducing sugar as the index. Following single factor experiment, the enzymatic hydrolysis conditions of agarose were optimized by four factors ( cellulase content, enzymolysis temperature, p H and enzymolysis time) Box-Behnken experiment design and response surface methodology ( RSM) , and a regression model was established for predicting the enzymatic hydrolysis yield at different levels of cellulase content, enzymolysis temperature, p H and enzymatic hydrolysis time. In addition, the enzymatic hydrolysate products were preliminary identified. The optimum enzymolysis parameter, including, the substrate content was 0.2%, the cellulase content was 1909.3 U/m L, the enzymolysis temperature was 50.0 ℃, the p H was 4.9, and the enzymolysis time was24.2 h. Under these conditions, the enzymolysis rate of agarose reached 29.58% ± 0.26%. The results of thin layer chromatography ( TLC) showed that the main products were agar oligosaccharides with polymerization degree of 2⁶.