Application of quantitative real-time PCR in meat and meat products

Quantitative real-time PCR as a technology for quantitative measurement of DNA has been widely used in the identification and quantitative analysis the species of meat products. Compared with the traditional or physical and chemical identification, quantitative real-time PCR has the advantages of high sensitivity and specificity, simple operation and time saving.The amount of products of the PCR cycle and types of amplified DNA fragments can be determined by the use of Taq Man probe and fluorescence dyes, then types and addition of meat is determined. Therefore, the technology can be applied to the detection of adulteration of meat and meat products safety.The principle of quantitative real-time PCR was introduced briefly, and application of quantitative real-time PCR was mainly reviewed in species identification and bacterial contamination of meat and meat products.Finally, the application of this technology in meat progress was prospected.